Part:BBa_K644000

Extracellular Domain of E-Cadherin (Mouse)

Cadherin is an adhesive transmembrane and calcium dependent protein that is expressed in many mammalian tissue cells and plays a role in keeping tissues together. It has a domain that anchors the protein to the cytoskeleton of the mammalian cells, an intercellular domain, and five extracellular domain repeats. With the presence of calcium ions, the extracellalur domains become rigid and are able to form homodimers with the same type of cadherin or form heterodimers with different types of cadherin, hence creating cell to cell adhesion.

• This E-cadherin only contains the extracellular domains of the protein.

We cloned E-cadherin into our plasmid pCTCON2 because pCTCON2 contained the Aga1 and Aga2 protein gene sequences which helped display our E-cadherin and other cloned proteins on the surface of S. cerevisiae. pCTCON2 also contains a Galactose promoter and our proteins were only displayed when S. cerevisiae was grown under the presence of galactose.

E-cadherin expressed on S. cerevisiae with Calcium ions

• all the pictures are of EBY100 S. cerevisiae transformed with E-cadherin grown under the presence of galactose.

E-cadherin expressed on S. cerevisiae without Calcium

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 285
  Illegal BamHI site found at 361
  Illegal BamHI site found at 477
  Illegal BamHI site found at 1401
  Illegal BamHI site found at 1703
  Illegal XhoI site found at 1085
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 405
  Illegal BsaI site found at 947